KRIBIOLISA CRM197 (Carrier Protein) Quantification ELISA

KRIBIOLISA CRM197 (Carrier Protein) Quantification ELISA

SKU: KBVH097

Rs 72,000.00

Enzyme Immunoassay for the estimation of KRIBIOLISA CRM197 (Carrier Protein) Quantification in serum, plasma, cell culture supernatant, buffers (including PBS 7.4) and other biological samples.

Assay Range: 6.25 ug/ml – 800 ug/ml | Sensitivity: 5 ug/ml

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

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All our kits are manufactured under ISO 13485.

The KRIBIOLISA CRM197 (Carrier Protein) Quantification ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of the biomarker – antigen/antibody. This kit has application for Carrier protein monitoring: conjugation development, consistency, comparability.

Additional information

Pack Type

1 x 96 wells, break-apart wells
Vaccine Development

Conjugate / Toxoid / Polysaccharide Vaccine
Vaccine Technology

Conjugate / Toxoid
Sample Type

Culture supernatant, vaccines and other biological preparations.
Assay Range

6.25 ug/ml – 800 ug/ml
Sensitivity

5 ug/ml
Interference

High levels of detergents/surfactants, organic solvents, strong chaotropes (e.g., urea/guanidine), extreme pH, or highly complex adjuvanted matrices may affect recovery and should be evaluated using spike-recovery and dilution linearity. For samples containing high antigen load or polysaccharide-conjugates, dilute in sample diluent to minimize matrix effects.
Principle Of Assay

This ELISA is a sandwich immunoassay . Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.
Specificity

This assay recognises CRM197 (Carrier Protein) Quantification
Detection Method

Colorimetric, Absorbance measured at 450nm
Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.
Validation Guideline (*request guideline document)

WHO guidance (TRS) for conjugate/toxoid vaccine evaluation; ICH Q2(R2)/Q14 for assay validation (as applicable).
Regulatory Status

For Research Use Only (RUO).
Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius
Storage Temperature

The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity.
ELISA Interpretation

Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.
Shelf Life Available

8 -10 months at time of shipping
Alternate Names / Synonyms

CRM197; Carrier Protein
Research Area

Immune molecule, Virology, Vaccine
ELISA Type

Sandwich ELISA, Double Antibody
Disclaimer

The data indicated herein with specifications are changed from time to time at time of production of the kit. We request you to confirm the specifications as per the most current IFU (instructions for use) accompanying the kit.
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