GENLISA Rabbit Rubella IgG Antibody ELISA

SKU: KBVR380

Rs 72,000.00

Enzyme Immunoassay for the estimation of Rubella IgG Antibodies in rabbit serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Range: Quantitative | Sensitivity: 10 ng/ml

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

 

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All our kits are manufactured under ISO 13485.

The GENLISA Rabbit Rubella IgG Antibody ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of the biomarker – antigen/antibody.

Additional information

Vaccine Development

Rubella Virus

Vaccine Technology

VLP / Recombinant

Product Catalog

Infectious Disease

Species Reactivity

Rabbit

Sample Type

buffer based solutions (pH7.4) and other biological samples., Cell Culture Supernatant, plasma, Serum

Assay Range

Quantitative

Standards Available

0, 15.6, 31.25; 62.5; 125; 250; 500; 1000; 2000 ng/ml

Sensitivity

10 ng/ml

Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.

Specificity

This assay recognises Rubella IgG Antibody

Principle Of Assay

This ELISA is a sandwich immunoassay. Antigen Protein is coated on 96 well plates. The Antibodies present in sample and standard bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of IgG Antibodies present in the standard or samples.

Detection Method

Colorimetric, Absorbance measured at 450nm

Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.

Validation Guideline (*request guideline document)

Platform expectations may vary; commonly referenced: ICH Q5A (viral safety) and ICH Q5D (cell substrates) where applicable, plus ICH Q2(R2)/Q14 for analytical methods.

Regulatory Status

For Research Use Only (RUO).

Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius

Storage Temperature

The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity.

ELISA Interpretation

Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.

Shelf Life Available

8 -10 months at time of shipping

Alternate Names / Synonyms

Human Papilloma Virus. HPV 18 L1; HPV18L1

Research Area

Immune molecule, Virology, Vaccine

Disclaimer

The data indicated herein with specifications are changed from time to time at time of production of the kit. We request you to confirm the specifications as per the most current IFU (instructions for use) accompanying the kit.