GENLISA Human Calprotectin (S100A8/S100A9 Heterodimer) ELISA

SKU: KBH6470

Rs 30,000.00

Enzyme Immunoassay for the estimation of Calprotectin (S100A8/S100A9 Heterodimer) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids as validated

Assay Range: 0.625 – 40 ng/ml | Sensitivity: 0.3 ng/ml

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

 

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All our kits are manufactured under ISO 13485.

Introduction: The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

Intended Use: The GENLISA Calprotectin (S100A8/S100A9 Heterodimer) ELISA kit is used as an analytical tool for quantitative determination of Calprotectin (S100A8/S100A9 Heterodimer) in serum, plasma and other biological samples.

Principle: The method employs sandwich ELISA technique. Antibody:HRP conjugate is added. After washing microwells in order to remove any non-specific binding, the Chromogenic substrate solution is added to microwells and color develops proportionally to the amount of Calprotectin (S100A8/S100A9 Heterodimer) in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Citation: The Monaldi Archives for Chest Disease – Chauhan NK, Rathore YS, Banerjee M, et al. Utility of pleural fluid biomarkers in differentiating malignant and benign pleural effusion: a cross-sectional study. Monaldi Arch Chest Dis doi: 10.4081/monaldi.2026.3731

 

Additional information

Pack Type

1 x 96 wells, break-apart

Species Reactivity

Human

Sample Type

Cell Culture Supernatant and other biological samples. Validated for citrated/EDTA Plasma only. Check with us if you wish validation data for CSF or tear fluids or any other specific matrices., plasma, Serum

Assay Range

0.625 – 40 ng/ml

Standards Available

0; 0.625; 1.25; 2.5; 5; 10; 20; 40 ng/ml

Sensitivity

0.3 ng/ml

Principle Of Assay

Double Antibody, Sandwich ELISA

Detection Method

Absorbance measured at 450nm, Colorimetric

Conjugate-Enzyme Reaction

and dynamic range, balancing speed, sensitivity

Regulatory Status

For Research Use Only (RUO).

Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius. Some components may be shipped to -20 degrees Celsius

Storage Temperature

if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results

ELISA Interpretation

4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, Quantitative Results Intepretation, to support interpretation of results.

Shelf Life Available

8 -10 months at time of shipping

Alternate Names / Synonyms

S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9; Calprotectin; S100A8/S100A9 Heterodimer

Research Area

Immune Molecule

Disclaimer

The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit.