Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.
Intended Use:
The GENLISA Chicken Heat Shock Protein 70, HSP70 ELISA kit is used as an analytical tool for quantitative determination of Chicken Heat Shock Protein 70, HSP70 in serum, plasma and other biological samples.
Principle:
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Chicken Heat Shock Protein 70, HSP70 present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin:HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Chicken Heat Shock Protein 70, HSP70 in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
